The utility model provides a blood collecting holder which is essentially consisting of a duplex puncture needle and a tube holder, with the puncture needle connected with a blood taking needle by a hose. The holder is in characterized in that the blood taking needle hub is provided a card to plug into the bayonet of the end of the holder. The utility model provides the fixation of the blood taking needle hub with the holder by rotating 90 degree during the clinic operation. Push the vacuum blood taking tube upward to pierce the sealing plug. The blood taking operation can be done by one hand, and releasing the tube after blood taking can allow needle withdrawal, which improve the blood collecting speed significantly, reduce the manufacture cost and thus is applicable to mass production and one-off use.